The basic analysis to assess male fertility is semen analysis performed twice with an interval of between two weeks and three months. Incorrect results are common among those exposed to toxicological factors (alcohol, smoking), temperature (varicocele), steroid and some other drugs.
Semen analysis is performed in accordance with the WHO recommendations („Laboratory manual for the Examination and processing of human semen 2010”). Our offer includes:
– Semen analysis with morphological assessment
– CASA – Computer Assisted Semen Analysis
– Analysis of sperm chromatin integrity using the SCA (Sperm Class Analyzer)
The basic analysis focuses on volume, appearance and time of liquefaction. Sperm count, their motility, vivacity and morphology is assessed. Moreover, the spermatogenetic cells, leucocytes and bacteria are assessed. Also the quality assessment of sperm chromatin may be performed – the sperm DNA fragmentation factor that informs about their capacity to fertilise both spontaneously and in vitro.
Current referential values according to WHO dated 2010 are presented below:
– Volume > 1.5 ml
– Concentration ≥ 15 mln/ml
– Total sperm count ≥ 39 mln
– Sperm motility ≥ 32% sperms with progressive motility or ≥ 40% sperms with progressive or non-progressive motility
– Sperm vivacity : ≥ 58% live spermatozoa
– Sperm morphology ≥4% correct morphology spermatozoa
Results below referential values may suggest the man’s fertility limitation. Similarly, correct results of semen analysis are not an indisputable proof of fertility.
Semen analysis may be performed in VitroLive in Szczecin, al. Wojska Polskiego 103.
Before collecting the sample, a 2-5 day-long period of sexual abstinence is required. The semen should be stored in a sterile container. If the sample was collected at home, it needs to be transported to the laboratory within 30-40 min, preserving human body temperature, i.e. ca. 36.6°C. Semen is collected by masturbation or during sexual intercourse to a special condom without spermicide (perforated condom).
Semen analysis with additional HBA (Hyaluronan Binding Assay) testing might be, in some clinical situations, a valuable addition to the basic semen analysis. It allows to assess the binding ability between the sperm and hyaluronan, and thereby the percentage of the sperms, able to fertilize an egg cell.
One of the indications to perform this test is idiopathic infertility (for which the cause is unknown). In case of couples with normal semen parameters in the basic test, who are getting prepared for an in-vitro fertilization procedure, the test might be helpful in taking the decision on the method of egg cell fertilization (classical IVF or ICSI).
The test cannot be performed if there are no motile sperms, as the basis for the test is an assessment of motile sperm activity.